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thermostable polymerases q5  (New England Biolabs)
99
New England Biolabs thermostable polymerases q5
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Thermostable Polymerases Q5, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thermostable+dna+polymerase/Q5+HF+DNA+Pol/pmc13601594-293-0-17
Average 99 stars, based on 1 article reviews
thermostable polymerases q5 - by Bioz Stars, 2026-11
99/100 stars
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blend taq  (Toyobo)
96
Toyobo blend taq
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Blend Taq, supplied by Toyobo, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thermostable+dna+polymerase/Blend+Taq/custom%40btq-101%4042142211
Average 96 stars, based on 1 article reviews
blend taq - by Bioz Stars, 2026-11
96/100 stars
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taq pol thermostable dna polymerase  (Jena Bioscience)
95
Jena Bioscience taq pol thermostable dna polymerase
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Taq Pol Thermostable Dna Polymerase, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thermostable+dna+polymerase/Taq+Polymerase/pmc13183530-182-50-55
Average 95 stars, based on 1 article reviews
taq pol thermostable dna polymerase - by Bioz Stars, 2026-11
95/100 stars
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thermostable dna polymerases  (New England Biolabs)
99
New England Biolabs thermostable dna polymerases
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Thermostable Dna Polymerases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thermostable+dna+polymerase/T4+DNA+Polymerase/us12571798-1364-50-81
Average 99 stars, based on 1 article reviews
thermostable dna polymerases - by Bioz Stars, 2026-11
99/100 stars
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thermostable dna polymerase q5  (New England Biolabs)
99
New England Biolabs thermostable dna polymerase q5
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Thermostable Dna Polymerase Q5, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thermostable+dna+polymerase/Q5+Hot+Start+High-Fidelity+DNA+Polymerase/pmc12828650-544-7-13
Average 99 stars, based on 1 article reviews
thermostable dna polymerase q5 - by Bioz Stars, 2026-11
99/100 stars
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thermostable t7 dna polymerase  (Amersham Pharmacia Biotech Ltd)
86
Amersham Pharmacia Biotech Ltd thermostable t7 dna polymerase
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Thermostable T7 Dna Polymerase, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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thermus aquaticus taq thermostable dna polymerase  (New England Biolabs)
98
New England Biolabs thermus aquaticus taq thermostable dna polymerase
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Thermus Aquaticus Taq Thermostable Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hs thermostable taq dna polymerase  (TaKaRa)
97
TaKaRa hs thermostable taq dna polymerase
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Hs Thermostable Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for polymerases with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and Q5 polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.

Journal: Nature Communications

Article Title: Quantifying replication fidelity of unnatural base pairs using nanopore sequencing

doi: 10.1038/s41467-026-76964-9

Figure Lengend Snippet: a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for polymerases with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and Q5 polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.

Article Snippet: Thermostable polymerases Q5 (M0491L), Phusion (M0530S), DeepVent (M0258S), DeepVent (exo-) (M0259S), and OneTaq (M0480S) were purchased from New England Biolabs (Ipswich, MA).

Techniques: Amplification, Transformation Assay, Concentration Assay, Sequencing, Two Tailed Test, Activity Assay, Derivative Assay